Table 2. Xyloglucan Structure Determined by Oligosaccharide Mass Profiling.
| Tissue | Plant Line | GXXG (953 m/z) | XXXG (1085 m/z) | XXLG/XLXG (1247 m/z) | XXLG/XLXG +OAc (1289 m/z) | XXFG (1393 m/z) | XXFG +OAc (1435 m/z) | XLFG (1555 m/z) | XLFG +OAc (1597 m/z) |
|---|---|---|---|---|---|---|---|---|---|
| Lower | Col-0 | 2.0 ± 0.2 | 32.5 ± 2.8 | 9.3 ± 0.7 | 1.8 ± 0.2 | 15.0 ± 0.9 | 14.1 ± 0.8 | 13.7 ± 1.6 | 11.5 ± 0.5 |
| Stem | uxt1-1 | 2.1 ± 0.3 | 33.2 ± 1.8 | 8.6 ± 0.6 | 1.4 ± 0.1 | 16.1 ± 0.5 | 14.1 ± 0.4 | 14.3 ± 1.1 | 10.3 ± 1.0 |
| uxt1-2 | 2.1 ± 0.1 | 35.0 ± 1.3 | 7.8 ± 0.2 | 1.4 ± 0.1 | 15.2 ± 0.2 | 14.4 ± 0.4 | 13.3 ± 0.3 | 10.9 ± 0.9 | |
| Upper | Col-0 | 3.2 ± 0.5 | 37.5 ± 0.9 | 13.0 ± 0.2 | 1.0 ± 0.1 | 19.0 ± 1.3 | 7.3 ± 0.3 | 15.5 ± 0.3 | 3.5 ± 0.3 |
| Stem | uxt1-1 | 2.7 ± 0.1 | 33.8 ± 0.7 | 12.1 ± 0.7 | 1.2 ± 0.1 | 20.7 ± 0.9 | 8.6 ± 1.1 | 16.1 ± 0.8 | 4.8 ± 0.2 |
| uxt1-2 | 2.9 ± 0.2 | 36.1 ± 1.4 | 12.4 ± 0.7 | 1.4 ± 0.1 | 20.1 ± 1.2 | 7.7 ± 0.5 | 15.0 ± 0.4 | 4.4 ± 0.3 |
Relative abundance of xyloglucan oligosaccharides in the upper and lower stem walls was determined by OLIMP. No significant differences (P < 0.05) were observed between the wild type and mutants. Data represent the mean (±se) of n = 3 independent biological replicates. The mass-to-charge ratio (m/z) is provided for each oligosaccharide; for nomenclature of the oligosaccharides, see Fry et al. (1993).