Figure 2.

Deletion Analysis of SULTR2;1 3′-Downstream Region.

(A) Effects of the 3′-deletions of the SULTR2;1 downstream region on Luc reporter activity under +S and –S conditions. Schematic representation of the 3′-deletions of the SULTR2;1 downstream region fused to the Luc gene (left), relative Luc activities (middle), and the –S/+S ratios of Luc activities (right) are described. Error bars in the middle panel denote the se of the mean. Asterisks indicate significant differences (Student’s t test; P < 0.05) between 2;1PLT1077 and other lines. The –S/+S ratios of Luc activities in each independent transgenic line were calculated and are shown as open circles. The geometric means of the –S/+S ratios are shown on the right with lowercase letters representing the significant differences (P < 0.05).

(B) Deletion analysis of the region between +372 and +361 from the translational termination codon of SULTR2;1. The deletion constructs of the SULTR2;1 downstream region (left) and the –S/+S ratios of Luc activities (right) are described as in (A).

T₂ progenies of five to seven independent transgenic lines from each construct were grown for 10 d on agar medium containing 1500 μM (+S, open bars in [A]) or 15 μM (–S, closed bars in [A]) of sulfate. Luc activities of root tissues from 25 plantlets were assayed as described in the Methods.