Figure 4.

The SULTR2;1 3′-Region Controls –S-Inducible Expression Independent of the Promoter.

Schematic representation of the promoter-Luc constructs with or without SULTR2;1 3′-region (left), average values of relative Luc activities (middle), and the –S/+S ratios of Luc activities (right) are described. T2 progenies of five to six independent transgenic lines from each construct were grown and analyzed as described in Figure 2. Error bars in the middle panel denote se. Asterisks in the middle panel indicate statistically significant differences (P < 0.05) between the plants harboring T_SULTR2;1 and T_NOS in the downstream of the same promoter. The –S/+S ratios of Luc activities in each independent transgenic line were calculated and are shown as closed circles. The geometric means of the –S/+S ratios are shown in the right graph with asterisks representing the significant differences (P < 0.01). P_35S, CaMV 35S promoter; P_SULTR1;1, 2453-bp upstream sequence from the translation initiation codon of SULTR1;1. Statistical analysis was performed using Student’s t test.