Figure 7.

T-DNA Insertion between Stop Codon and SURE21A/SURE21B Disrupts –S-Inducible Expression of SULTR2;1 in Roots.

(A) Structure of SULTR2;1 genomic region and T-DNA insertion sites (triangles) in the knockout (tKO) mutants. Thick black bars represent exons. In the tKO mutants in Col-0 and Ws backgrounds, T-DNAs are inserted in positions +306 and +415 bp from the translational termination codon of SULTR2;1, as indicated by white and gray triangles, respectively.

(B) Gel images of real-time RT-PCR products showing SULTR2;1 and UBQ2 (UBIQUITIN2) mRNA accumulations. The wild type (Col-0 and Ws) and tKO mutants were grown for 10 d on +S and –S media.

(C) Relative transcript levels of SULTR2;1 quantified by real-time RT-PCR. UBQ2 transcript levels were used for normalization. SULTR2;1 transcript levels in wild-type roots under –S and wild-type shoots under +S are shown as 100%. Error bars denote se (n = 3). P values of the Student’s t tests of the comparisons between the wild-type and tKO plants on either +S or –S conditions are indicated above the tKO data with asterisks (*0.05 ≤ P < 0.1 and **P < 0.05).