Figure 2.

Storage Protein Profile in Maturing, Dry, and Germinating nhx5 nhx6 Seeds.

(A) Seed protein profile (Coomassie blue-stained gel) collected at different developmental stages: immature (Imm), dry, and either 3 or 6 d after germination. The wild type (WT) and nhx5 nhx6 (KO) were loaded in alternate lanes.

(B) Immunoblot of protein extracts (from [A]) probed using anti-12S and anti-2S antibodies. In nhx5 nhx6 (F37), an accumulation of pro forms (immature) of both 12S (top blot) and 2S (bottom blot) storage proteins is evident. Mature forms (2S and 12S) persisted in nhx5 nhx6 seeds 3 d after germination.