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. 2015 Mar 27;27(4):1228–1250. doi: 10.1105/tpc.114.135731

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© 2015 American Society of Plant Biologists. All rights reserved.

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Figure 6. — The GPI-Anchored PD Protein, PDCB1, Associates with the DIM Fraction, while the PD Protein, PDLP1, Does Not.

(A) Isolation of DIMs from Arabidopsis suspension cells. Six fractions of equal volume were collected from the top to the bottom of the sucrose gradient (F1 to F6).

(B) Protein quantification of collected fractions F1 to F6 shows that the DIM fraction (F3) contains ∼15% of total proteins, while the DSM fractions (F5 and F6) contain 75% of total proteins.

(C) Immunoblot of fractions F1 to F6. The GPI-anchored protein PDCB1 is highly enriched in the DIM fraction (F3), while PDLP1 associates with the DSM fractions (F5 and F6).