Figure 5.

Narrowing Down the Target Sequences of the ncc1 and ncc2 Mutations.

(A) Schematic representation of the mutant petA genes, presented as in Figure 3A. The “F” indicates the position of the introduced frame shifts, while gray and white rectangles show translated and untranslated petA sequences, respectively.

(B) and (C) petA transcript accumulation in tetrad progeny from crosses {f₁₄₅St} × ncc2 (B) and {f₄₂St} × ncc2 (C). Loading controls: psbD (B) and atpA (C).

(D) Accumulation of atpA-hybridizing transcripts in wild-type and ncc1 strains transformed with the Δf::α_Tr chimera (Figure 3A). The probe hybridizes with the chimeric transcript, either alone (Δf::α_Tr) or cotranscribed with the aadA cassette (asterisk) and with endogenous mono- (circle) and dicistronic (square) atpA mRNA.