Figure 6.

ProPEAMT1:GUS Expression in Roots and Leaves of Wild-Type and pah1 pah2 Plants.

GUS assays were performed on roots and leaves of 4-week-old wild-type and DM (pah1 pah2) plants containing ProPEAMT1:GUS constructs (A) without (B) or with (C) a functional uORF in the 5′UTR. Single insertion site transgenic lines were first created by transforming the wild type, and the T-DNAs were then introduced into the pah1 pah2 background by crossing. Activities are the mean ± se of measurements on tissue from four separate plants. Asterisk denotes a significant difference from the wild type (P < 0.05).