Figure 2.

BBX19 Is a COP1-Dependent and HY5-Independent Suppressor of Photomorphogenesis.

(A) and (B) Hypocotyl phenotypes of 5-d-old Col-0, OE44, HY5-OE, and OE44/HY5-OE seedlings grown under LD conditions (A) and measurements of their hypocotyl lengths (B).

(C) and (D) Relative expression levels (C) and the corresponding protein levels (D) of HY5 examined by protein gel blot analyses using HY5-specific antibody in Col-0, OE44, and Ri323 lines. The Ponceau S (PS) staining shows equal protein loadings. Total RNA was extracted from these genotypes and subjected to quantitative PCR analysis. The transcript levels of HY5 were normalized to At4g34270 (T1P41-like family protein) and At4g26410 (M3E9) measured in the same samples. Data are mean fold differences ± sd of three biological replicates each with three technical repeats.

(E) and (F) Hypocotyl phenotypes of 5-d-old Col-0, OE44, cop1, and OE44/cop1 seedlings grown under LD conditions (E) and measurements of their hypocotyl lengths (F).

For (B) and (F), data are means ± sd; n ≥ 90. Letters above the bars indicate significant differences as determined by Tukey’s HSD method (P < 0.05).