Figure 4.

The Rice Gene LOC_Os12g33610 Encodes a Functional Tyrosine Aminomutase That Produces β-Tyrosine.

(A) Accumulation of β-tyrosine in N. benthamiana with transient expression of LOC_Os12g33610. Control, empty vector; ND, not detected. Mean ± se of n = 3.

(B) HPLC chromatogram from in vitro tyrosine aminomutase activity assays. Black, β-tyrosine standard; red, Tris buffer + tyrosine + enzyme; blue, Tris buffer + tyrosine; green, Tris buffer+ enzyme. Inset picture shows an immunoblot. L, ladder; CP, crude protein; PP, His-tag purified protein.

(C) to (E) Accumulation β-tyrosine in vitro increases with the time of the reaction (C), the enzyme concentration (D), and the substrate concentration (E).

(F) and (G) The temperature optimum for the reaction is 29°C (F) and the pH optimum is 10.5 (G). Mean ± se of n = 3.