Figure 4.

Effects of Light Treatments on the Localization of NPH3 Proteins.

(A) Localization of NPH3 proteins without red light pretreatment. Dark-grown transgenic nph3 plants harboring 35S_pro:YFP-NPH3 were stimulated with blue light at 0.17 μmol m^–2 s^–1, and YFP signals were observed with a microscope at the indicated times after the onset of blue light irradiation. Bar = 200 μm.

(B) and (C) Effects of red light pretreatment on the localization of NPH3 proteins. The transgenic plants were pretreated with (C) or without (B) overhead red light and then stimulated with blue light at 0.17 μmol m^–2 s^–1. Time 0 corresponds to the onset of blue light irradiation. Bars = 100 μm.

(D) Localization of NPH3 proteins in the phot1 mutant. Dark-grown phot1 mutants harboring 35S_pro:YFP-NPH3 were stimulated with blue light at 0.17 μmol m^–2 s^–1. Bar = 50 μm.

(E) Localization of NPH3 proteins in the rpt2-2 mutant. Dark-grown rpt2-2 mutants harboring 35S_pro:YFP-NPH3 were stimulated with blue light at 0.17 μmol m^–2 s^–1. Bar = 50 μm.

(F) Distribution of YFP fluorescence. Dark-grown Columbia seedlings harboring 35S_pro:YFP were stimulated with blue light at 0.17 μmol m^–2 s^–1. Bar = 50 μm.

(G) Quantification of YFP granules. The nph3, phot1, and rpt2-2 mutants harboring 35S_pro:YFP-NPH3 were pretreated with or without overhead red light (RL) and then stimulated with blue light at 0.17 μmol m^–2 s^–1. Time 0 corresponds to the onset of blue light irradiation. The number of YFP granules of >1 μm diameter was counted after blue light irradiation. The data shown are means ± se from 8 to 18 seedlings. Double asterisks indicate statistically significant differences compared with red light pretreatment (Student’s t test, P < 0.01).