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. 2015 Sep 3;16(1):662. doi: 10.1186/s12864-015-1831-7

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© Papsdorf et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of different polyQ stretches in yeast cells on phosphate metabolism. a ³¹P-NMR spectra of yeast cells expressing Q₀-YFP (top), Q₃₀-YFP (middle) or Q₅₆-YFP (bottom). Yeasts were resuspended to an OD₅₉₅ = 300 in phosphate free buffer. Chemical shift positions are listed in Additional file 7, 1: inorganic phosphate, 2: polyphosphate (end of chain), 3: polyphosphate (internal). b Exophosphatase activity was monitored upon transferring yeasts to phosphate free media. Q₀-YFP is depicted in black, Q₃₀-YFP in blue and Q₅₆-YFP in red. Results were obtained from three independent experiments. Values were normalized for better comparability. Mean and SEM are depicted. Statistical significance is indicated with a star (α = 0.05)