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. Author manuscript; available in PMC: 2015 Sep 3.
Published in final edited form as: J Neurochem. 2005 Feb;92(4):730–738. doi: 10.1111/j.1471-4159.2004.02900.x

Fig.2. The enhancer of the ACT gene is functional in astrocytes but not in hepatoma cells.

Fig.2

Human astrocytes or HepG2 cells were transfected with plasmids pΔACTCAT (ENH-ACTCAT) or pENHPCICAT and β-galactosidase expression vector as internal control for transfection efficiency. One day after transfection cells were stimulated with IL-1 (10 ng/ml) or TNF (10 ng/ml) in the presence of 1 μM dexamethasone, cultured for another 24 hours, and harvested. CAT activities were normalized to β-galactosidase activities (four separate analysis), and are shown as a fold induction with control cultures equal to 1.