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. Author manuscript; available in PMC: 2015 Sep 3.
Published in final edited form as: J Neurochem. 2005 Feb;92(4):730–738. doi: 10.1111/j.1471-4159.2004.02900.x

Fig. 3. Activation of NF–κB in human astrocytes, astrocytoma and hepatoma cells.

Fig. 3

A. Human astrocytes, U373-MG and HepG2 cells were stimulated with IL-1, OSM, or Dex for 20 minutes. Nuclear extracts were prepared and binding was analyzed using the NF–κB oligonucleotide.

B. Cells were stimulated with IL-1 and DEX for the indicated time periods, nuclear extracts were prepared, and binding analyzed using the NF–κB oligonucleotide. C. Cells were stimulated with IL-1 and DEX for either 20 min or 24 hours, nuclear cell extracts were prepared, incubated with anti-p65, anti-p50, anti-p52, anti-RelB, anti-c-Rel or normal rabbit serum (NRS) and binding was analyzed using the NF–κB oligonucleotide. D. HepG2 cells were stimulated with IL-1 and DEX for the indicated time periods, nuclear and cellular extracts were prepared and analyzed by Western blotting using anti-p65 antibodies. Representative results of three separate experiments are shown.