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. 2015 Sep 3;11:29. doi: 10.1186/s13005-015-0086-5

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© Del Angel-Mosqueda et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Cell culture and flow cytometry analysis of isolated dental pulp stem cells (DPSCs). a Representative phase-contrast micrographs shows unsorted-cells derived from human dental pulp tissue after 14 days of cell culture. b–d CD105⁺ magnetically-sorted DPSCs cultured in α-MEM without osteogenic induction. Morphologically, cells appear as typical fibroblastic and spindle shape during 3 passages. Original magnification 10x, scale bar =100 μm. e Flow cytometric analysis presented as histograms that show cell fluorescence intensity on the horizontal axis and cell frequency distribution on the vertical axis. Percentage results show positive expression to immunophenotype associated with mesenchymal stem cell (MSC) lineage as well as a lack of expression for hematopoietic markers