Table 1.
Strains and plasmids used in this study
| Strains or plasmids | Description | Reference or source |
|---|---|---|
| Escherichia coli | ||
| E. coli top10 | F-mcrAΔ (mrr-hsdRMS-mcrBC) ψ80lacZΔM15Δ lacX74 nupG recA1 araD139Δ (ara-leu) 7697 galE15 galK 16 rpsL (StrR) end A1λ- | Invitrogen (Shanghai) |
| B. amyloliquefaciens | ||
| SQR9 | Wild-type isolate | [44] |
| ΔdhaS | ΔdhaS::Cmr | This study |
| ΔpatB | ΔpatB::Cmr | This study |
| ΔyclB | ΔyclB::Cmr | This study |
| ΔyclC | ΔyclC::Cmr | This study |
| ΔyhcX | ΔyhcX::Cmr | This study |
| ΔysnE | ΔysnE::Cmr | This study |
| patB-E | B. amyloliquefaciens SQR9 with pUBC19-P43-patB (kanr) | This study |
| yclC-E | B. amyloliquefaciens SQR9 with pUBC19-P43-yclC (kanr) | This study |
| dhaS-E | B. amyloliquefaciens SQR9 with pUBC19-P43-dhaS (kanr) | This study |
| SQR9-E | B. amyloliquefaciens SQR9 with pUBC19-P43-BCS (kanr) | This study |
| SQR9-CK | B. amyloliquefaciens SQR9 with pUBC19-P43 (kanr) | This study |
| B. subtilis | ||
| 168 | Wild-type isolate | Lab strain |
| 168-E | B. subtilis 168 with pUBC19-P43-BCS (kanr) | This study |
| 168-CK | B. subtilis 168 with pUBC19-P43 (kanr) | This study |
| Plasmids | ||
| pUBC19 | Ampr Kmr; B.subtilis-E. coli shuttle vector | [47] |
| pNW33n | Cmr | [46] |
| pUBC19-P43 | pUBC19 containing P43 promoter | This study |
| pUBC19-P43-patB | pUBC19 containing P43 promoter and patB | This study |
| pUBC19-P43-yclC | pUBC19 containing P43 promoter and yclC | This study |
| pUBC19-P43-dhaS | pUBC19 containing P43 promoter and dhaS | This study |
| pUBC19-P43-E | pUBC19 containing P43 promoter and fusion fragments for expression | This study |
| pUBC19-dhaS | pUBC19 containing dhaS | This study |
| pUBC19-yclC | pUBC19 containing yclC | This study |
| pUBC19-ysnE | pUBC19 containing ysnE | This study |
Cm r chloroamphenicol resistant, kan r kanamycin resistant, Amp r Ampicillin resistant