Fig. 3.

Spontaneous cardiac differentiation of monolayer cultured miPSCs on 3D PCL nanofibrous scaffolds. a Schematic of the protocol for the spontaneous CM differentiation of Oct4-GFP⁺ miPSC monolayers cultured without MEF feeder layers in vitro. b SEM images of the cell morphologies of purified miPSCs on gelatin-coated 3D PCL nanofibrous scaffolds (top) and in gelatin-coated TCPs (bottom) after 1 day of monolayer culture. 5000× magnification; scale bar, 10.0 μm. c Images of the spontaneous differentiation of miPSC monolayers cultured on 3D PCL nanofibrous scaffolds and in TCPs at different time points; scale bars, 100 μm. d RT-PCR analysis of gene expression of spontaneously differentiated miPSC monolayer cells over 15 days of differentiation on 3D PCL nanofibrous scaffolds. e Immunofluorescence images acquired on day 15 of spontaneous differentiation on 3D PCL nanofibrous scaffolds identifying both cTnT and MLC2a with DAPI for nuclei; scale bars, 100 μm