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. 2015 Jul 7;5(15):3023–3030. doi: 10.1002/ece3.1575

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© 2015 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Transmission electron microscopy showing spermatozoal attachment in the oviduct of Chinese soft-shelled turtle. (A) Many spermatozoa (S) were embedded among cilia (Ci) and inserted into the apical hollowness of the ciliated cell (CC). (B) A spermatozoon inserted its head (H) into the hollowness which was surrounded by no lysosome in ciliated cell. (C) Spermatozoa were present at the opening of gland conduit. (D) Cross section of the spermatozoa head in the hollowness of ciliated cell and the spermatozoa midpiece among cilia. basal body (B), secretory cell (SC), mitochondrion (Mi). Scale bar = 2 μm (A, C) and 1 μm (B, D).