Fig. 3.

Comparison of content of each metabolite between SDT and SD rats. a Volcano plot of metabolome data on SDT and SD rats at 12 weeks of age. x axis shows log₂(fold change calculated by the value of SDT divided by the value of SD) and y axis shows −log₁₀(Welch’s t test p-value) between SDT and SD rats. Each dot represents one metabolite. Dotted lines shows 1.2 fold change and p = 0.01. Names of metabolite exhibiting significant differences (p < 0.01 and fold change >1.2) between SDT and SD rats are shown. b Longitudinal changes in metabolites showing significant differences between SDT and SD rats at 12 weeks of age (n = 8–11 each). Content of metabolite was expressed as intensity of each metabolite relative to that of internal standard. The data are expressed as means without SEM for clarity. Welch’s t test was used for evaluation of statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001