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Graves' orbitopathy fibroblasts demonstrate myogenic and neuronal lineage differentiation. Graves' orbitopathy fibroblasts (lines HO1-3) were induced toward myogenic (A–C) and neuronal (G–I) differentiation using specific media or kept in control medium under the same conditions (“undifferentiated” [D–F] and [J–K], respectively). (A–F) Cells were immunostained for α-SMA (red) and DAPI (blue). (G–L) Cells were immunostained for β-III Tubulin (green) and DAPI (blue). Scale bar: 100 μm.
