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. 2015 Aug 28;8:2319–2327. doi: 10.2147/OTT.S88661

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© 2015 Jia et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Decreased sensitivity of breast cancer cells to cisplatin by KLF4. KLF4 expression in breast cancer cells infected with the indicated lentivirus for 72 hours was detected by Western blot and normalized using β-actin.

Notes: (A) MCF-7 cells were infected with pWPTS-KLF4 (Lenti-KLF4) or pWPTS-GFP (Lenti-GFP) lentivirus. (B) MDA-MB-231 cells were infected with KLF4 shRNA (shKLF4) or negative control (shNC) lentivirus vector. (C) Comparison of cisplatin cytotoxicity in MCF-7 cells infected with pWPTS-KLF4 or pWPTS-GFP by the MTT assay. (D) Comparison of cisplatin cytotoxicity in MDA-MB-231 cells infected with shKLF4-1, shKLF4-2, or shNC by the MTT assay. Data are presented as the means ± SD (n=3). *P<0.05; **P<0.01.

Abbreviations: ALDH, aldehyde dehydrogenase; KLF4, Krüppel-like factor 4; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; shRNA, short hairpin RNA; GFP, green fluorescent protein.