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. 2015 Sep 3;15:176. doi: 10.1186/s12866-015-0492-7

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© Guldimann et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Immunofluorescence stained confocal images of bacteria in infected brain-slices. a: Delineation of an infection focus (yellow line). L. monocytogenes are stained in red. The surface area covered by L. monocytogenes was drawn and calculated using the Fluoview software (Olympus FV10-ASW Version 03.01.01.09) Magnification 20x. b: Representative double-immunofluorescence of a L. monocytogenes infected brain-slice. The vast majority of bacteria are found within microglia. Left: Microglia are stained with CD68 in green. Center: L. monocytogenes in red. Right: Overlay (bar = 40 μm)