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. 2015 Sep 3;10:46. doi: 10.1186/s13062-015-0077-2

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© Jaksik et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Individual steps of a microarray experiment. After isolation of sample mRNA (1) synthesis of cDNA (complementary DNA, step) chains begins with addition of oligo(dT) primers (2), then cDNA is amplified, producing cRNA (complementary RNA), which is labelled with biotin (3) and later fragmented (4). After such preparation sample cRNA is ready for hybridization with microarray probes (5) and ready for the final staining process (6)