Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Problems detected by different control probe-sets and their possible reasonsa
| Housekeeping genes | Poly-A spike | Bacterial spike | Possible reason |
|---|---|---|---|
| error | ok | ok | Poor quality of the mRNA analyzed |
| error | error | ok | Problems during amplification/labeling |
| error | error | error | Problems during hybridization/washing |
| ok | ok | error | Inaccurate preparation of bacterial spike |
| ok | error | ok | Inaccurate preparation of bacterial poly-A spike |
aOther possible combinations of errors rarely occur in practice
