Table 2.
Product yields (Y) of engineered E. coli strains expressing the synthetic pathway
| Strain name | Deletions | Plasmids | YBiomass (g/g) | YEG (mol/mol) | YGA (mol/mol) | YAcetate (mol/mol) | YFormate (mol/mol) |
|---|---|---|---|---|---|---|---|
| Pen877 | pEXT20-khkC-aldoB | 0.11 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 1.07 ± 0.02 | 0.20 ± 0.01 | |
| Pen205 | ΔxylB | pEXT20-khkC-aldoB | 0.14 ± 0.00 | 0.45 ± 0.01 | 0.09 ± 0.00 | 0.00 ± 0.00 | 0.14 ± 0.01 |
| Pen885 | ΔxylB | pEXT20-khkC-aldoB + pACT3-empty | 0.14 ± 0.00 | 0.33 ± 0.01 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.17 ± 0.01 |
| Pen222 | ΔxylB | pEXT20-khkC-aldoB + pACT3-gldA | 0.15 ± 0.01 | 0.37 ± 0.00 | 0.00 ± 0.00 | 0.01 ± 0.00 | 0.13 ± 0.03 |
| Pen223 | ΔxylB | pEXT20-khkC-aldoB + pACT3-yqhD | 0.18 ± 0.03 | 0.33 ± 0.00 | 0.03 ± 0.03 | 0.00 ± 0.00 | 0.22 ± 0.00 |
| Pen644 | ΔxylB | pEXT20-khkC-aldoB + pACT3-fucO | 0.14 ± 0.01 | 0.36 ± 0.01 | 0.06 ± 0.00 | 0.01 ± 0.00 | 0.18 ± 0.00 |
| Pen334 | ΔxylB ΔyqhD | pEXT20-khkC-aldoB | 0.14 ± 0.00 | 0.07 ± 0.01 | 0.04 ± 0.00 | 0.02 ± 0.00 | 0.01 ± 0.00 |
| Pen325 | ΔxylB ΔaldA | pEXT20-khkC-aldoB | 0.12 ± 0.01 | 0.88 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.02 ± 0.02 |
| Pen361 | ΔxylB ΔaldA ΔglcD | pEXT20-khkC-aldoB | 0.10 ± 0.00 | 0.87 ± 0.00 | 0.02 ± 0.00 | 0.00 ± 0.00 | 0.02 ± 0.00 |
| Pen332 | ΔxylB ΔaldA | pEXT20-khkC-aldoB + pACT3-gldA | 0.08 ± 0.00 | 0.52 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| Pen333 | ΔxylB ΔaldA | pEXT20-khkC-aldoB + pACT3-yqhD | 0.11 ± 0.01 | 0.90 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.01 | 0.01 ± 0.00 |
| Pen641 | ΔxylB ΔaldA | pEXT20-khkC-aldoB + pACT3-fucO | 0.16 ± 0.01 | 0.94 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.01 | 0.07 ± 0.01 |
Data is presented as means ± standard deviations of at least two independent experiments. For strains Pen334 and 332 only ~50 % of the initially present (d)-xylose were consumed. All experiments were performed in 250 mL shake flasks filled with 50 mL medium and shaken at 200 rpm.