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. 2015 Sep 4;12:27. doi: 10.1186/s12989-015-0102-8

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© Disdier et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — 2Representative images of immunofluorescence expression of tight junction protein occludin (red) and endothelial cell marker vonWillenbrand factor (vWF, green) in the cerebral microvasculature of (a) control rats, or tissues collected from rats treated with 1 mg/kg TiO₂ (IV) at (b) 5 min; (c) 6 h; (d) 24 h; or (e) 28 days post-treatment. Yellow fluorescence indicates overlay (co-localization) of occludin and vWF. Arrows indicate areas of increased yellow fluorescence. Graph in (f) shows analysis of relative fluorescence of overlay images ± SD. * P < 0.050 compared to controls