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. 2015 Sep 3;15:179. doi: 10.1186/s12862-015-0450-x

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© Buscà et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — ERK1 and ERK2 protein appear first in bony vertebrates (Bichir). Western-blot analysis of samples from brain, spinal cord or cultured cells: immunoblots were incubated with polyclonal anti-ERK antibody (ERK1 and ERK2; panels a, b, c) or anti-phosphoERK antibody (pERK1 and pERK2; panels d, e, f). Taxonomic names are listed in Additional file 7. Predicted MW sizes of vertebrate ERKs range from 41.7 kDa for lamprey’s ERK to 44.1 for zebrafish’s ERK1. SC: spinal cord; CE: cerebellum; OP: optic lobe. Positions of ERK1, ERK2, phospho-ERK1 (pERK1) and phospho-ERK2 (pERK2) are indicated on the sides