Table 1. L. infantum Alba-domain protein putative partners identified by co-immunoprecipitation and LC-MS/MS analysis.
| TriTrypDB ID | Identified Proteins | Peptide No. a (Probability score 99.9%) | |||
|---|---|---|---|---|---|
| L. infantum promastigotes | L. infantum amastigotes | ||||
| Alba1-HA | HA-Alba3 | Alba1-HA | HA-Alba3 | ||
| LinJ.34.2410 | hypothetical protein, conserved | LiAlba3 | 16 b | 15 | 15 | 16 |
| LinJ.13.0270 | hypothetical protein, conserved | LiAlba1 | 11 | 7 | 8 | 6 |
| LinJ.35.5360 | polyadenylate-binding protein 1, putative |PABP1 | 4 | 3 | 7 | 3 |
| LinJ.35.4200 | poly(A)-binding protein 2,poly(a) binding protein, putative |PABP2 | 12 | 15 | 17 | 12 |
| LinJ.25.0080 | poly(A)-binding protein 3 |PABP3 | 3 | 6 | 7 | 7 |
| LinJ.32.0410 | ATP-dependent RNA helicase, putative | Homolog of Dbp1 | 3 | 4 | 12 | 10 |
| LinJ.32.0790 | RNA binding protein, putative | NRBD | 4 | 3 | 4 | 6 |
| LinJ.21.0490 | hypothetical protein, conserved |NTF2-like | 2 | 3 | 9 | 9 |
| LinJ.27.1220 | hypothetical protein, conserved | ZC3H41 | 2 | 13 | 9 | |
| LinJ.26.1220 | heat shock protein 70-related protein | 2 | 2 | 9 | 4 |
| LinJ.36.3010 | 40S ribosomal protein S24e | 4 | 3 | 3 | |
| LinJ.35.0400 | 40S ribosomal protein S3A, putative | 2 | 3 | 2 | |
| LinJ.36.5240 | 40S ribosomal protein SA, putative | 4 | 2 | ||
| LinJ.28.1050 | 40S ribosomal protein S14 | 2 | 3 | 3 | |
| LinJ.21.2150 | 40S ribosomal protein S6, putative | 3 | 2 | 3 | |
| LinJ.07.0550 | 60S ribosomal protein L7a, putative | 3 | 2 | 2 | 3 |
| LinJ.29.1160 | ribosomal protein L1a, putative | 3 | 3 | ||
| LinJ.35.2240 | RNA-binding protein, putative | DRBD2 | 7 | 4 | ||
| LinJ.18.0300 | hypothetical protein, conserved |NTF2-like | 2 | |||
| LinJ.25.0550 | hypothetical protein SCD6.10 | Rap-55 | 2 | 1 | ||
| LinJ.22.1370 | 40S ribosomal protein L14, putative | 3 | |||
| LinJ.28.2750 | 40S ribosomal protein S17, putative | 3 | 3 | ||
| LinJ.34.2620 | 40S ribosomal protein S19 protein, putative | 2 | 2 | ||
| LinJ.15.1010 | 40S ribosomal protein S3, putative | 6 | 6 | ||
| LinJ.13.1120 | 40S ribosomal protein S4, putative | 7 | 4 | ||
| LinJ.11.0960 | 40S ribosomal protein S5 | 3 | |||
| LinJ.04.0460 | 60S ribosomal protein L11 (L5, L16) | 3 | |||
| LinJ.29.2570 | 60S ribosomal protein L13, putative | 2 | 3 | ||
| LinJ.32.4050 | 60S ribosomal protein L2, putative | 4 | |||
| LinJ.06.0590 | 60S ribosomal protein L23a, putative | 2 | 2 | ||
| LinJ.24.2140 | 60S ribosomal protein L26, putative | 3 | |||
| LinJ.35.1870 | 60S ribosomal protein L5, putative | 3 | 2 | ||
| LinJ.30.3390 | 60S ribosomal protein L9, putative | 4 | 4 | ||
| LinJ.01.0430 | ribosomal protein S7, putative | 4 | 3 | ||
| LinJ.30.2570 | reticulon domain protein, 22 potentially aggravating protein | 2 | |||
| LinJ.18.0590 | RNA binding protein, putative | Leish. specific | 2 | |||
aOnly proteins identified with a minimum of two peptides and a probability of 99.9% to correspond to the correct protein were included here. Identified proteins were grouped into different GO categories based on their predicted function. From those proteins, the ones presented in the upper portion of the Table associate with both LiAlba1 and LiAlba3 in L. infantum promastigotes and amastigotes, those in the middle part of the Table associate with LiAlba1 and LiAlba3 only in amastigotes, and one protein was found to interact with LiAlba1 in promastigotes only. The raw data of peptide and spectrum reports are provided in S2 Table.
bEach of the three co-IP experiments was run in triplicate. Only proteins identified in two out of the three replicates are presented here, and the number of unique peptides for each protein is representative of three experiments. The maximum number of peptides obtained is indicated here.