Table 2. List of the most representative components of the flagellar structure and RNA metabolism enriched in the L. infantum flagellum proteome.
| Gene ID | Identified Proteins | MWkDa | Pro (No. peptides) | Diff 8h (No. peptides) | |
|---|---|---|---|---|---|
| Paraflagellar rod components | LinJ.29.1880 (+1) | paraflagellar rod protein 1D, putative | 69 | 41–19 | 16–19 |
| LinJ.16.1510 | paraflagellar rod protein 2C | 69 | 32–21 | 17–23 | |
| LinJ.36.4440 | paraflagellar rod component, putative | 123 | 20–10 | 1–6 | |
| LinJ.09.1390 | paraflagellar rod component, putative | 68 | 14–5 | 7–8 | |
| LinJ.07.0470 | paraflagellar rod component, putative (PFC3) | Tb | 89 | 21–4 | 3–6 | |
| LinJ.36.6130 | paraflagellar rod component, putative | 86 | 15–10 | 2–9 | |
| LinJ.05.0040 | paraflagellar rod component par4, putative | 68 | 12–3 | 2–3 | |
| LinJ.02.0280 | paraflagellar rod component, putative (PFC11) | Tb. | 73 | 7–2 | 0–3 | |
| LinJ.27.1750 | paraflagellar rod protein-like protein | 89 | 10–1 | 0–3 | |
| LinJ.19.0520 | paraflagellar rod component, putative (PFC15) | Tb | 58 | 7–2 | 0–4 | |
| LinJ.36.5010 | paraflagellar rod component, putative | 34 | 4–3 | 0–3 | |
| Components of motile flagella | LinJ.27.0720 | hypothetical protein, conserved | TbCMF2 | 85 | 22–11 | 9–14 |
| LinJ.08.1030 | hypothetical protein, conserved | TbCMF3 | 97 | 21–4 | 2–2 | |
| LinJ.28.1210 | hypothetical protein, conserved | TbCMF15 | 68 | 11–6 | 3–2 | |
| LinJ.13.1580 | hypothetical protein, conserved | TbCMF22 | 104 | 12–5 | 0–4 | |
| LinJ.30.3700 | hypothetical protein, conserved | TbCMF46 | 64 | 11–2 | 1–3 | |
| LinJ.27.2090 | hypothetical protein, conserved | TbCMF5 | 174 | 9–7 | 0–0 | |
| Radial spoke components | LinJ.13.0290 | flagellar radial spoke protein, putative | 67 | 10–10 | 4–5 |
| LinJ.08.0900 | radial spoke protein RSP2, putative | Tb | 63 | 13–2 | 3–4 | |
| LinJ.27.2530 | radial spoke protein 3, putative | 42 | 6–4 | 5–3 | |
| LinJ.33.2630 | radial spoke protein RSP9, putative | 36 | 7–2 | 3–3 | |
| LinJ.03.0950 | radial spoke protein RSP10, putative | Tb. | 36 | 9–4 | 1–3 | |
| LinJ.29.0690 | flagellar radial spoke protein-like, putative | 76 | 5–3 | 0–1 | |
| Actin & Tubulin | LinJ.08.1290 (+1) | beta-tubulin | 50 | 28–25 | 27–24 |
| LinJ.13.0330 (+1) | alpha-tubulin | 50 | 34–20 | 21–20 | |
| LinJ.04.1250 | actin | 42 | 7–3 | 1–3 | |
| Dyneins | LinJ.25.1010 | dynein heavy chain, putative | 537 | 22–6 | 1–18 |
| LinJ.13.1390 | dynein heavy chain, putative | 529 | 17–8 | 0–12 | |
| LinJ.32.1120 | dynein, putative | 68 | 4–4 | 4–6 | |
| LinJ.24.0270 | dynein intermediate-chain-like protein | 75 | 8–3 | 1–3 | |
| LinJ.32.3050 | outer dynein arm docking complex protein | 70 | 5–3 | 0–6 | |
| LinJ.33.2770 | dynein intermediate chain, putative | 115 | 10–3 | 0–4 | |
| LinJ.26.1100 | dynein arm light chain, axonemal, putative | Tb | 50 | 8–3 | 1–1 | |
| Other flagellar components | LinJ.05.1080 | hypothetical protein, conserved | axoneme Tb | 80 | 13–8 | 6–9 |
| LinJ.14.1300 | hypothetical protein, conserved | Flagellum Tb | 99 | 22–7 | 0–4 | |
| LinJ.11.0810 | hypothetical protein, conserved | axoneme) Tb | 104 | 10–7 | 5–9 | |
| LinJ.20.1350 | small myristoylated protein-1, putative | 15 | 1–4 | 4–3 | |
| LinJ.24.2060 | STOP axonemal protein, putative | 30 | 10–4 | 4–1 | |
| LinJ.20.1450 | axoneme central apparatus protein, putative | 55 | 5–5 | 2–7 | |
| LinJ.10.1280 | flagellar protofilament ribbon protein-like protein | 47 | 14–3 | 1–0 | |
| LinJ.32.4020 | myosin XXI | 119 | 8–1 | 2–6 | |
| LinJ.32.3350 | hypothetical protein, conserved | FLAM7 | 292 | 10–7 | 0–6 | |
| LinJ.31.1240 | vacuolar-type proton translocating pyrophosphatase 1 | 83 | 7–4 | 5–4 | |
| LinJ.35.5310 | hypothetical protein, conserved | 39 | 6–3 | 2–4 | |
| LinJ.17.0970 | META domain containing protein, putative | 48 | 8–1 | 1–3 | |
| LinJ.29.2310 | GTP-binding protein, putative | dynamin | 78 | 3–0 | 9–0 | |
| RNA metabolism | LinJ.01.0790 (+1) | eukaryotic initiation factor 4a, putative | 45 | 7–8 | 10–9 |
| LinJ.32.0410 | ATP-dependent RNA helicase, putative | 67 | 16–8 | 17–12 | |
| LinJ.21.1820 | RNA helicase, putative | 59 | 4–2 | 6–4 | |
| LinJ.35.0370 | ATP-dependent DEAD-box RNA helicase, putative | 46 | 3–0 | 6–6 | |
| LinJ.35.3150 | ATP-dependent RNA helicase, putative | 101 | 2–0 | 7–4 | |
| LinJ.07.0130 | ATP-dependent DEAD/H RNA helicase, putative | 53 | 0–0 | 4–3 | |
| LinJ.35.4200 | poly(A)-binding protein 2, putative | 65 | 5–0 | 7–2 | |
| LinJ.25.0080 | poly(A)-binding protein 3, putative | 61 | 1–0 | 2–0 | |
| LinJ.35.2240 | RNA binding protein, putative | 30 | 1–0 | 2–2 | |
| LinJ.34.2870 | ZFP family member, putative (ZC3H10) | Tb | 64 | 4–3 | 1–2 |
Proteome analyses were performed in duplicate for L. infantum promastigotes and differentiating amastigotes (8 h). Only proteins identified with an average of 2 peptides in 2 independent experiments and a probability of >95.0% to correspond to the correct protein were included here. A more complete list of identified proteins (with 2 peptides in at least one experiment) is presented in S3 Table. The raw data of the peptide and spectrum reports are provided in S4 Table. Because Leishmania databases are still poorly annotated for flagellum components, we also analyzed the Trypanosoma orthologs to include proteins previously described as flagellar components [30] (see gene names or comments on TritrypDB.org). The reference to the T. brucei (Tb) orthologs is shown by the presence of the | separator.