Fig 2. Coverage of exon-capture sequences by exon size, intron size and on-target/off-target homoeologue.

(A) Read depth of exon-capture sequences increases with exon size. (B) Read depth of small (<100bp) internal exons (those flanked by two introns) decreases with flanking intron size. (C) The on-target homoeologues have better coverage by the mapped reads than the off-target homoeologues. In the box-and-whisker plots, the top and bottom boundaries of the blue boxes indicate the 75^th and 25^th centiles, respectively, the whiskers indicate the maxima and minima, the central bars are the median values and the red spots show the means.