Table 4. Validation of candidate SNPs by KASP marker analysis.
Criterion | Number/ Frequency | KASP assays | Validated SNPs | Validated (%) |
---|---|---|---|---|
Variant reads | 4 | 25 | 1 | 4 |
5 | 17 | 3 | 18 | |
6 | 14 | 2 | 14 | |
7 | 14 | 3 | 21 | |
8 | 10 | 5 | 50 | |
9 | 12 | 9 | 75 | |
>10 | 35 | 29 | 83 | |
Allele frequency | 0.2 | 51 | 2 | 4 |
0.4 | 27 | 13 | 48 | |
0.6 | 18 | 9 | 50 | |
0.8 | 3 | 2 | 67 | |
1 | 31 | 27 | 87 |
Candidate SNPs were classified by number of supporting variant reads or by allele frequency and validated by KASP assay.