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. 2015 Sep 3;10(9):e0136817. doi: 10.1371/journal.pone.0136817

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© 2015 Yong et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Her2^+/- pregnant females (bred to Her2^+/- males) were sacrificed at days E14.5 to >E15 and embryos harvested. A-O) Representative images of fetuses. P) Amniotic sacs taken from a single female showing distinct yellow appearance of two fetuses in situ. Sample number refers to the litter from which the mice were taken. Mice within the same sample number were taken at the same time point. Genotypes of mice indicate homozygous for the Her2 transgene (+/+), heterozygous for the Her2 transgene (+/-) or wild type (-/-). Genomic DNA was taken and analysed for the expression of both Pds5b and Her2 by PCR to confirm zygosity (see Fig 5).