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. Author manuscript; available in PMC: 2016 Sep 3.
Published in final edited form as: Cell Rep. 2015 Mar 5;10(9):1459–1466. doi: 10.1016/j.celrep.2015.02.020

Figure 3. mGluR-LTD Persists in the Absence of Translation if the Proteasome Is Inhibited.

Figure 3

(A) Field recordings from acute hippocampal slices (mouse and rat) treated with either 60 μM CHX (blue) or 60 μM CHX and 5 μM MG132 (CHX/MG, orange) for 1 hr. Dashed boxes show time of DHPG application (mouse, 50 μM, 5 min; rat, 100 μM, 6 min). Scale bars indicate 10 ms and 0.25 mV. (Right) Average fEPSP slope during the last 5 min of recording indicates inhibiting the proteasome rescues mGluR-LTD blocked by translational inhibition ([mouse] VEH, 61.3 ± 2.6; CHX, 94.7 ± 4.7; CHX+MG132, 66.9 ± 3.9; [rat] VEH, 71.6 ± 5.2; CHX, 91.2 ± 3.3; CHX+MG132, 67.7 ± 6.0).

(B) Concurrent field (black) and whole-cell recordings (red) of mGluR-LTD (50 μM DHPG, 5 min) from mouse slices. (Left) CHX (60 μM) included in the perfusate blocked LTD (WC, 109 ± 12; field, 110 ± 6). (Middle) Lactacystin (1 μM) included in the recording pipette, with vehicle in the perfusate resulted in no significant difference in the amount of LTD between the field and whole-cell recordings (WC, 68.5 ± 7.7; field 67.7 ± 6.9). (Right) Lactacystin (1 μM) in the recording pipette and 60 μM CHX in bath blocked LTD in the field recordings, but not in the whole-cell recordings (WC, 68.7 ± 11.5; field, 108.8 ± 10.3). Scale bars indicate 0.25 mV/50 pA and 10 ms.

Summary data consist of mean ± SEM.