Fig. 2.

Accumulation curves and their 95 % confidence intervals for Aceria tosichella cryptic species resulting from an intensive sampling scheme. As the sampling effort increased, the total number of recorded genetic lineages rose and reached an asymptote that corresponds to an estimated size of the total lineage pool (including those lineages yet unknown). It was recently found (Chiu et al. 2014) that this type of estimate gives only the lower limit of the richness parameter. Thus, it is likely that the “true” number of genetic lineages within the Aceria tosichella complex (provided that sampling is restricted to the same geographic region, range of habitats and host species) will be higher. The panels represent different nonparametric methods of estimation of accumulation curves: S—no. of biotypes, Bootstrap—bootstrap estimator, described in Smith and van Belle (1984). Calculations were made in R using the function “specpool” from the package “vegan” (Oksanen et al. 2013)