Fig. 1.

Schematic maps of TMEM33 cDNA, and predicted open reading frame and amino acid sequence of TMEM33. a Maps of the complete human TMEM33 (alias SHINC3) cDNA and overlapping partial clones are shown. The gray box represents the coding region and the black boxes represent the 5′- and 3′- untranslated regions of the cDNA sequence. Italicized numbers represent the original lengths of the overlapping clones in the GenBank database. The NCBI BLAST program [43] was used to identify the overlapping sequences and deduce the full-length sequence of TMEM33 cDNA. b Predicted open reading frame (366–1109 bp) and amino acid sequence alignment of TMEM33 protein (247 aa). Locations of three transmembrane helices (32–52 aa, 101–121 aa, and 156–176 aa, underlined), a phosphorylation site (T65, red) and two ubiquitination sites (K148 and K221, blue) are shown