Fig. 7.

Exogenous expression of TMEM33 in MCF-7 breast cancer cells is associated with constitutive activation of the PERK and IRE1α axes of the UPR signaling. MCF-7 cells were transiently transfected with Myc-TMEM33 or pCR3.1 control vector. Adherent and floating cells were collected 24 h post-transfection. Cells were lysed in RIPA lysis buffer supplemented with protease inhibitor (Roche), 10 mM glycerophosphate, 1 mM sodium orthovanadate, 5 mM pyrophosphate, and 1 mM PMSF. Cell lysates (10 μg protein) were immunoblotted with indicated antibodies (all 1:1000 dilution)