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. 2015 Sep 4;6:686. doi: 10.3389/fpls.2015.00686

FIGURE 1.

FIGURE 1

Induction of AtERF15 by infection with Pst DC3000 and Botrytis cinerea and by treatments with salicylic acid and methyl jasmonate. (A) Induction of AtERF15 by Pst DC3000. (B) Induction of AtERF15 by B. cinerea. Four-week-old Col-0 plants were inoculated by vacuum infiltration with Pst DC3000 (OD600 = 0.002) (A), by spraying with spores of B. cinerea (2 × 105 spores/ml) (B), or by treatment with corresponding solution (10 mM MgCl2) for Pst DC3000 and buffer (4% maltose and 1% peptone) for B. cinerea as mock controls. (C) Induction of AtERF15 by SA and MeJA. Four-week-old Col-0 plants were treated by foliar spraying with 1 mM salicylic acid (SA), 100 μM jasmonic acid (MeJA), or 0.1% ethanol as mock controls. The inoculated or treated leaves were collected at indicated times and expression of AtERF15 was analyzed through qRT-PCR. Data were normalized with the transcript level of AtActin as an internal control and relative expression levels were shown as folds of the level of AtActin. Data presented are the means ± SD from three independent experiments and above the columns indicate significant differences at p < 0.05 level between the inoculated/treated plants and mock control plants.