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. 2015 Sep 4;6:686. doi: 10.3389/fpls.2015.00686

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Copyright © 2015 Zhang, Huang, Dai, Liu, Hong, Tian, Huang, Cao, Li and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transactivation activity and nuclear localization of AtERF15 protein. (A) AtERF15 has transactivation activity. Yeast cells carrying pBD-AtERF15, pBD-GAL4 (a positive control) or pBD empty vector (a negative control) were grown on SD/Trp^- plates for 3 days at 30°C and activity of β-galactosidase was detected by addition of x-α-gal. (B) AtERF15 is localized in nucleus. Agrobacteria carrying pFGC-Egfp-AtERF15 or pFGC-Egfp empty vector were infiltrated into leaves of Nicotinana benthamiana plants expressing a red nucleus marker protein RFP-H2B and leaf samples were collected at 24 h after infiltration for observation under a confocal laser scanning microscope. Images were taken in dark field for GFP and RFP, bright field for cell morphology and in combination, respectively.