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. 2015 Sep 4;6:926. doi: 10.3389/fmicb.2015.00926

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Copyright © 2015 García, Alcaraz, Franco and Passerini de Rossi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CLSM analysis of biofilms from S. maltophilia K279a and its Fur mutant. Biofilms were grown in glass LabTek chambers, in TSB and TSB supplemented with 200 μM Dip for 48 h. Then, biofilms were stained with Syto9 and analyzed by CSLM, using a 40X water immersion objective lens. (A) Central panels represent the x–y plane, and the top and right side panels represent the x–z and y–z planes, respectively. Scale bars represent 20 μm. (B) Three-dimensional projections of biofilm structure were reconstructed using the Image J program. The images are representative of those obtained on three independent occasions.