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Microscopy detection of matrix exopolysaccharides in S. maltophilia biofilms. K279a and F60 biofilms formed on borosilicate coverslips in TSB (upper panel) or TSB-Dip (lower panel) for 48 h were stained with calcofluor white and examined by epifluorescence microscopy. The polysaccharide matrix fluoresces blue under the DAPI light filter. Calcofluor stains cells as discrete points while EPS is visible as a continuous sheet. Biofilms were viewed at 400X magnification.
