Table 3.
Relative vector competence of Culex restuans, Cx. pipiens, and Aedes triseriatus for LACV following oral exposure
| Species and group number | Blood meal LACV titer | DPE | Sample size (n) | % Non-disseminated (95% CI) | % Disseminated (95% CI) | % Transmitting (95% CI) |
|---|---|---|---|---|---|---|
| Cx. restuans 1 | 2.9 × 107 | 10 | 16 | 12 (0–0.4) | 31 (0.1–0.6) | 31 (0.1–0.6) |
| Cx. restuans 2 | 1.8 × 107 | 10 | 5 | 0 (0–0.5) | 0 (0–0.5) | 0 (0–0.5) |
| Cx. restuans 3 | 8.5 × 106 | 10 | 12 | 0 (0–0.3) | 0 (0–0.3) | 0 (0–0.3) |
| Cx. restuans 1 | 2.9 × 107 | 14 | 14 | 0 (0–0.2) | 0 (0–0.2) | 0 (0–0.2) |
| Cx. restuans 2 | 1.8 × 107 | 14 | 7 | 14 (0–0.5) | 0 (0–0.4) | 0 (0–0.4) |
| Cx. restuans 3 | 8.5 × 106 | 14 | 13 | 0 (0–0.3) | 8 (0–0.4) | 8 (0–0.4) |
| Total Cx. restuans | – | – | 67 | 5 (0–0.1) | 9 (0–0.2) | 9 (0–0.2) |
| Cx. pipiens 1 | 1.3 × 107 | 10 | 13 | 15 (0–0.4) | 8 (0–0.4) | 8 (0–0.4) |
| Cx. pipiens 2 | 1.3 × 107 | 10 | 21 | 5 (0–0.3) | 14 (0–0.4) | 9 (0–0.3) |
| Cx. pipiens 1 | 1.3 × 107 | 14 | 18 | 5 (0–0.3) | 0 (0–0.2) | 0 (0–0.2) |
| Cx. pipiens 2 | 1.3 × 107 | 14 | 18 | 17 (0–0.4) | 17 (0–0.4) | 0 (0–0.2) |
| Total Cx. pipiens | – | – | 70 | 10 (0.1–0.2) | 10 (0–0.2) | 4 (0–0.1) |
| Ae. triseriatus | 1.4 × 107 | 10 | 21 | 10 (0–0.3) | 57 (0.4–0.7) | 57 (0.4–0.7) |
| Ae. triseriatus | 1.4 × 107 | 14 | 21 | 33 (0.2–0.5) | 33 (0.2–0.5) | 24 (0.1–0.5) |
| Total Ae. triseriatus | – | – | 42 | 21 (0.1–0.4) | 45 (0.3–0.6) | 40 (0.3–0.5) |
CI = confidence interval; DPE = days postexposure; LACV = La Crosse virus.
Mosquitoes were collected at 10 and 14 DPE. If virus was recovered from the abdomen but not from the legs or expectorate, the mosquito was considered to have a non-disseminated infection. If virus was recovered from the abdomen and legs, it was classified as a disseminated infection. Mosquitoes with virus-positive salivary expectorate were classified as transmitting.