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. 2015 Sep 2;93(3):591–595. doi: 10.4269/ajtmh.15-0276

Figure 2.

Figure 2.

Entamoeba histolytica recombinase polymerase amplification (RPA) in lateral flow (LF) strips. Entamoeba histolytica, E. dispar, Cryptosporidium, and Giardia deoxyribonucleic acid (DNA) and no template (NT) were used to evaluate the specificity of E. histolytica RPA in LF strips. RPA detects E. histolytica DNA but not E. dispar (left). RPA detects E. histolytica using DNA of E. histolytica spiked with DNA of Cryptosporidium and Giardia (Right). Cryptosporidium and Giardia DNA alone were negatives (Right). White arrows indicate control bands for the assay and black arrows show detection zone of RPA positives.