Table 1.

RPA in DNA from clinical samples. DNA obtained from patients positives for Entamoeba histolytica, E. dispar, E moshkovskii, or negatives were analyzed by RPA in LF strips

Sample	ELISA/PCR	RPA	Microscopy/symptoms
1	Entamoeba moshkovskii	Negative	Cysts in stools
2	Negative	Negative	Negative
3	Negative	Negative	Negative
4	Negative	Negative	Negative
5	E. histolytica	E. histolytica	Cysts in stools, tenesmus, stools with mucus and occasional blood, acute diarrhea, abdominal pain
6	E. moshkovskii	Negative	Cysts in stools
7	Negative	Negative	Negative
8	Negative	Negative	Negative
9	E. histolytica	E. histolytica	Cysts in stools, stools with mucus diarrhea, abdominal pain
10	Negative	Negative	Negative
11	E. moshkovskii	Negative	Cysts in stools
12	Negative	Negative	Negative
13	E. moshkovskii	Negative	Cysts in stools
14	Negative	Negative	Negative
15	E. moshkovskii/E.dispar	Negative	Cysts in stools
16	E. moshkovskii	Negative	Cyst in stools
17	E. dispar	Negative	Cyst in stools
18	E. histolytica	E. histolytica	Cysts in stools, soft stools with mucus and occasional blood, abdominal pain
19	E. dispar	Negative	Cysts in stools
20	E. histolytica	E. histolytica	Cysts in stools, tenesmus, abdominal pain
21	E. dispar	Negative	Cysts in stools
22	E. dispar	Negative	Cysts in stools
23	Negative	Negative	Negative, helminths infection
24	Negative	Negative	Negative
25	E. moshkovskii	Negative	Cyst in stools
26	E. dispar	Negative	Cyst in stools
27	E. moshkovskii	Negative	Cyst in stools
28	Negative	Negative	Negative
29	Negative	Negative	Negative
30	E. histolytica	E. histolytica	Cysts in stools, acute diarrhea, soft stools with mucus and occasional blood, tenesmus, abdominal pain
31	E. dispar	Negative	Cysts in stools
32	Negative	Negative	Negative
Total positives	5	5
Total negatives	27	27

The RPA results were obtained using 5 μL of template from each clinical sample. DNA = deoxyribonucleic acid; ELISA = enzyme-linked immunosorbent assay; LF = lateral flow; PCR = polymerase chain reaction; RPA = recombinase polymerase amplification.