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. 2015 Sep 4;5:13576. doi: 10.1038/srep13576

Figure 4. Evaluation of cell viability with knockdown of target genes.

Figure 4

Knockdown of PTPRF, PRKAR2B, MAP4K3, and RICTOR was enabled by siRNA transfection as described in the Materials and Methods. (A) At 48 h post-transfection, cells were treated with doxorubicin (5 mg/L), epirubicin (5 mg/L), and 5-fluorouracil (50 μM) for 24 h, and then cell viability was measured by WST-1 assay. (B) At 48 h post-transfection, cells were incubated with EGF or serum-free media for 24 h, and then cell viability was measured by WST-1 assay. Data are presented as means ± SEM, n = 4. Each group (such as Control, Doxorubicin, and EGF) was compared by one-way ANOVA with Tukey’s post hoc test for multiple comparisons. For all groups, P < 0.0001 in ANOVA, and asterisks indicate significant differences by Tukey’s post hoc test (*P < 0.05, **P < 0.01, ***P < 0.001) compared with the siControl. Levels of significance for comparisons between the control and EGF groups in (B) were determined using Student’s t-test (N.S. indicates not significant).