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. 2015 Sep 4;5:13576. doi: 10.1038/srep13576

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Knockdown of PTPRF, PRKAR2B, MAP4K3, and RICTOR was enabled by siRNA transfection, and cell migration was measured by wound-healing assay as described in the Materials and Methods. (A) At 48 h post-transfection, MDA-MB-231 cells were subjected to a wound-healing assay. Inside the horizontal axis (white dashed line) represents the wounded region. Images were captured at 0, 12, and 24 h using phase contrast microscopy. (B) Cell migration was quantified in terms of two features using CellProfiler. Black bar indicates the ratio of the occupied area with migrated cells to the total wounded area. White bar indicates the total number of migrated cells in the wounded region.