Table 1. Affinity assays for TNF α or RMP16 binding with HSA, MSA, hTNFR I and hTNFR II.
| SPR (KD, M) |
ITC (KD, M) |
|||
|---|---|---|---|---|
| RMP16 | TNF α | RMP16 | TNF α | |
| HSA | 8.75 × 10−7 | 8.22 × 10−7 | ||
| MSA | 9.25 × 10−7 | 8.94 × 10−7 | ||
| hTNFR I | 2.13 × 10−8 | 4.79 × 10−9 | 2.04 × 10−8 | 4.26 × 10−9 |
| hTNFR II | 8.64 × 10−6 | 1.31 × 10−9 | 8.17 × 10−6 | 1.19 × 10−9 |
HSA: Human serum albumin; MSA: Mouse serum albumin; hTNFRI: human TNFRI; hTNFRII: human TNFRII. The binding affinities were measured by Surface Plasmon Resonance (SPR) and isothermal titration calorimetry (ITC).