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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2014 Dec 24;91(3):621–630. doi: 10.1016/j.ijrobp.2014.10.047

Figure 5.

Figure 5

(A) Western blot analysis of HIF-1α in HT1080 cells transduced with HIF-1α shRNA (sh.HIF-1α) or scrambled (Scr) shRNA. Actin blot serves as loading control. (B) Proliferation of HT1080 cells after RT (6 Gy) and/or transduction with HIF-1α shRNA (sh.HIF-1a) or scrambled shRNA (sh.Scr). (C) Proliferation of four STS cell lines 3 days after RT and/or low dose doxorubicin (0.005 μM). (D) Colony formation of four STS cell lines in normoxia and hypoxia after RT (0, 2, 4 and 6 Gy), and/or low dose doxorubicin (0.005 μM). All experiments were performed in normoxia and hypoxia. *p<0.05 compared to all other groups.