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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Endocr Relat Cancer. 2015 Jul 23;22(5):777–792. doi: 10.1530/ERC-14-0302

Figure 9. Downstream effects of HDAC1 and HDAC6.

Figure 9

A. QPCR of RhoB, p21 and BIM in HDAC1 and HDAC6 silenced THJ-16T cells show that all 3 mRNAs are upregulated. B. Western blot analysis of nontarget, HDAC1 789, HDAC6 3384 and HDAC6 3840 shRNA clones were examined for differential downstream targets effects. β-actin was used as a loading control. C. Nontarget and HDAC shRNA cells were selected for 24 h and then plated in triplicate in 12-well culture plates at 2 × 104 cells/well for 72 h. Data was plotted as cell number ± S.D. * indicated p<0.05 was considered statistically significant when compared to nontarget control. D. After 36 h selection, cells were plated for cell death analysis and incubated for 72 h prior to flow cytometry with propidium iodide.