Table I.
Amino acid sequence and relative hydrolysis rates of newly synthesized substrates.
# | Peptide Substrate |
Relative Hydrolysis Rate |
---|---|---|
c0 | GSSKLQ | 100.0 |
c1 | GSSKLH | 170.6 |
c2 | GSSKLA | 31.5 |
c3 | GSSKYQ | 256.9 |
c4 | GSSKPQ | 7.2 |
c5 | GSSSLQ | 52.6 |
c6 | GSSALQ | 43.2 |
c7 | GSAKLQ | 207.2 |
c8 | GSSKLq | 14.2 |
c9 | GSSKlQ | 10.7 |
c10 | GSSkLQ | 5.0 |
c11 | GSsKLQ | 103.8 |
c12 | GsSKLQ | 104.1 |
The peptide GSSKLQ (c0) was used as a reference to calculate relative hydrolysis rates. The single amino acid difference between a peptide and the reference sequence is indicated by bold Italic font at the corresponding position. The small underlined letter in c8–12 peptides indicates d-amino acid at that position.