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. 2015 Sep 4;5:13617. doi: 10.1038/srep13617

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Copyright © 2015, Macmillan Publishers Limited

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Since the outbreak isolates shared an indistinguishable genome content, a representative outbreak-associated isolate XY1 was used to evaluate the viral replication. The isolate XY1 was recovered from the first case (Index case A) from the Xiangyang outbreak, and the on-outbreak-associated isolate CDC228 was recovered from a sporadic ARD patient from Xiangyang City. Virus titers were determined in Hep-2 cells by measuring the 50% tissue culture infectious dose (TCID₅₀).